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POLYMERASE CHAIN REACTION DETECTION OF AVIAN LEUKOSIS VIRUS DNA IN VACCINES USED IN POULTRY Rev Salud Anim.
Acevedo,Ana María; Rodríguez,Edisleidy; Uffo,Odalys; Relova,Damarys; Noda,Julia; Díaz de Arce,Heidy.
Avian leukosis viruses (ALV) provoke a variety of trasmissible bening and malign tumoral diseases affecting birds. Chickens are affected by six subgroups of ALV designs A, B, C, D, E and J of more recent world dissemination. These viruses are potential contaminants of live vaccines used in poultry. In order to research the presence of DNA from ALVs as contaminants of viral commercial vaccines to be used in poultry, different Marek´s disease vaccines were screened by a reported polymerase chain reaction (PCR) assay designed to detect all subgroups of ALVs. DNA samples extracted from seven vaccines were submitted to PCR using primers for a conserved region of env gene of HPRS-103. ALV sequences were detected in seven samples (100%). The methodology employed...
Tipo: Journal article Palavras-chave: Avian leukosis virus (ALV); Polymerase chain reaction (PCR); Vaccine; Contamination; Poultry.
Ano: 2009 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2009000100011
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Antimicrobial action of the essential oil of Lippia gracilis Schauer BABT
Albuquerque,Cynthia Cavalcanti de; Camara,Terezinha Rangel; Mariano,Rosa de Lima Ramos; Willadino,Lilia; Marcelino Júnior,Cristiano; Ulisses,Cláudia.
Antimicrobial activity of the essential oil (EO) of Lippia gracilis Schauer over fungi and heliconia endophytic bacteria was evaluated. For the fungi Geotrichum candidum; Trichoderma viride; Torula herbarum; Paecillomyces sp.; Fusicoccum sp.; P. aeruginens; Curvularia lunata; Aspergillus nidulans; A. flavus; and A. niger mycelial discs were inoculated in PDA medium with oil (0; 420; 440; 460; 480 e 500 µL L-1). The inhibition percentage in 420 µL L-1 was 100% for all fungi, except for C. lunata and A. niger, which was 95.58% and 89.40% , respectively. In other experiment, a suspension of the Salmonella choleraceuis-diarizonae, Enterobacter asburiae, Bacillus thuringiensis, B. pumilus, B. cereus, Klebsiella pneumoniae, and E. hormaechei was individually...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Micropropagation; Contamination; Monoterpenes.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500001
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Etude sanitaire du Havre du Payré (1989-1993) et qualité des coquillages commercialisés (1980-1991) ArchiMer
Allenou, Jean-pierre; Pezeron, Annie; Dumont, Francoise; Menanteau, Chantal.
Le Havre du Payré situé au sud des Sables d'Olonne abrite une zone ostréicole de production modeste (1 000 tonnes en 1992). Le réseau de surveillance du milieu marin mis en place par l'IFREMER en 1989 a mis en évidence des épisodes de contamination important sur ce site. Les niveaux atteints en 1992 ont entraîné la fermeture de la zone. Une dynamique s'est alors mise en place entre les diverses Administrations et l'IFREMER a réalisé un suivi intensif sur les trois derniers mois de l'année 1993.
Tipo: Text Palavras-chave: Hâvre du Payré; Coliformes fécaux; Surveillance microbiologique; Etablissement d'expédition; Contamination.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00081/19212/16807.pdf
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L'étain et les organoétains en milieu marin. Biogéochimie et écotoxicologie. ArchiMer
Alzieu, Claude.
This report synthesizes the knowledge on the biogeochemical cycle, bioconcentration processes and ecotoxicity of tin and organotins in the marine environment. Concentrations levels, biomethylation mechanisms and exchanges between sediments and water are assessed. Emphasis is given on tributyltin sublethal effects, toxicity mechanisms and no effect levels for the most sensitive organisms. Regulations on the use of organotins as antifoulants in paints are examined. Conclusions and recommendations for environmental management and research are given.
Tipo: Text Palavras-chave: Étain; Biogéochimie; Contamination; Écotoxicologie.; Tin; Biogeochemistry; Contamination; Ecotoxicology.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/00332/44314/43883.pdf
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Silver European eels health in Mediterranean habitats ArchiMer
Amilhat, Elsa; Fazio, Geraldine; Simon, Gael; Manetti, Marc; Paris, Severine; Delahaut, Laurence; Farrugio, Henri; Lecomte-finiger, Raymonde; Sasal, Pierre; Faliex, Elisabeth.
The degradation in the quality of silver eel and their health could have been a major factor in the collapse of the European eel (Anguilla anguilla) population. However, the health status of the spawners has been poorly studied until now. This study evaluated the quality of migrant male silver eels from four Mediterranean habitats in France presenting different degrees of contamination: Canet-Saint-Nazaire, Salses-Leucate and Bages-Sigean lagoons and La Berre River. We considered pathogens including Anguillicoloides crassus and EVEX virus and the concentration of chemical contaminants including PCBs, OCs and heavy metals. Our study results revealed different patterns of pollution and infection in the four habitats, with high individual variability. No...
Tipo: Text Palavras-chave: Anguilla anguilla; Mediterranean lagoons; Silver eel; Contamination; Spawner quality; Pathogens.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00171/28252/26543.pdf
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Qualité microbiologique des coquillages destinés à la consommation humaine : contamination des coquillages par Escherichia coli. Sous-région marine Golfe de Gascogne. Evaluation initiale DCSMM. ArchiMer
Amouroux, Isabelle.
Tipo: Text Palavras-chave: Golfe de Gascogne; Coquillages; Escherichia coli; Contamination; Réglementation; Qualité microbiologique; Evolution.
Ano: 2012 URL: https://archimer.ifremer.fr/doc/00329/44060/43631.pdf
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Qualité microbiologique des coquillages destinés à la consommation humaine : contamination des coquillages par Escherichia coli. Sous-région marine Manche - Mer du Nord. Evaluation initiale DCSMM. ArchiMer
Amouroux, Isabelle.
Tipo: Text Palavras-chave: Manche; Mer du Nord; Coquillages; Contamination; Escherichia coli; Réglementation; Qualité microbiologique; Evolution.
Ano: 2012 URL: https://archimer.ifremer.fr/doc/00328/43873/43447.pdf
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Bioaccumulation of mercury, cadmium, zinc, chromium, and lead in muscle, liver, and spleen tissues of a large commercially valuable catfish species from Brazil Anais da ABC (AABC)
Arantes,Fábio P.; Savassi,Lourenço A.; Santos,Hélio B.; Gomes,Marcos V.T.; Bazzoli,Nilo.
The increasing amounts of heavy metals entering aquatic environments can result in high accumulation levels of these contaminants in fish and their consumers, which pose a serious risk to ecosystems and human health. We investigated the concentrations of mercury (Hg), cadmium (Cd), zinc (Zn), chromium (Cr), and lead (Pb) in muscle, liver, and spleen tissues of Pseudoplatystoma corruscans specimens collected from two sites on the Paraopeba River, Brazil. The level of heavy metals concentrations in the tissues was often higher in viscera (i.e. liver and spleen) than in muscle, and thus, the viscera should not be considered for human consumption. Correlations between metal concentrations and fish size were not significant. Although the levels of muscle...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Contamination; Freshwater fish; Heavy metal; Human health.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100137
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Etat de la contamination du Bassin d'Arcachon par les insecticides et les herbicides sur la période 2005-2006. Impact environnemental ArchiMer
Auby, Isabelle; Bocquene, Gilles; Quiniou, Francoise; Dreno, Jean-paul.
Within the framework of the study on cup oyster breeding in the Arcachon Basin (IFREMER's SURGIBA Programme, co-financed by Europe, the Regional Council of Aquitaine, the Regional Council of Gironde and the Arcachon Basin's inter-communal Syndicate), some pesticides have been researched in the main waterways and in the waters of several of the Basin's zones (Figure 1), during the summer months (June to September) from 1999 to 2003 inclusive (Auby and Maurer, 2004). The presence of a certain number of molecules, mainly from herbicides, was found in the waters and it has generally been possible to identify their source. Certain substances come from agricultural uses or park maintenance; others are from nautical sources (anti-fouling paint for protecting the...
Tipo: Text Palavras-chave: France; Arcachon basin; Hydrology; Chlorpyfiros; Chlorpyfiros; Insecticide; Weedkiller; Contamination; Marine environment.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/rapport-2398.pdf
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Dépistage des sources de pollutions microbiologiques en Seudre amont et dans le bassin de Marennes Oléron ArchiMer
Auger, Christian; Courtois, Olivier; Kantin, Roger; Masson, Daniel; Piquet, Jean-come; Roesberg, Didier.
The oyster culture basin of Marennes - Oléron is characterized by the tastiness of its oysters and the good quality of its waters, and thus of its shellfish. On that account, the quasi-totality of the production areas are ranked A for non-burrowing shellfishes, thus confirming the privileged character of the basin. This microbiological and chemical quality contributing to the reputation of the basin's shellfish farms needs to be preserved. In this purpose, the Regional Shellfish Culture Section of Marennes - Oléron (SRC) approached the Ifremer in order to continue the monitoring of the microbiological quality carried out on different production areas susceptible to be affected by some bacteriological contaminations leading to a deterioration of the...
Tipo: Text Palavras-chave: Contrôle; Contamination; Seudre; Bassin de Marennes Oléron; Surveillance; Pollution microbiologique.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/rapport-6304.PDF
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Environment and immunomodulation : effects of the soluble fraction of Norwegian heavy fuel on the immune system of Pacifc oyster, Crassostrea gigas ArchiMer
Bado-nilles, Anne; Auffret, Michel; Quentel, Claire; Le Floch, Stéphane; Arzel, Jacqueline; Gagnaire, Beatrice; Renault, Tristan; Thomas-guyon, Hélène.
Estuaries and coastal areas are often subjected to much pollution, including pesticides and polycyclic aromatic hydrocarbons (PAHs) produced by anthropic activities. Bay of Marennes-Oléron is an estuarine coastal zone in which there is a high level of aquacultural production and this has adverse effects in aquatic organisms. In fact, PAHs, which are widely spread throughout aquatic ecosystems, have a low polarity and so there is a clear tendency to bioaccumulate in marine organisms (1). The harmful effects of such contaminants on the organism's immune system, leading to a decreased resistance to pathogens, is a relatively new area of research and it still poorly understood. The aim of the present work was to detect the impact in vivo of PAHs on the immune...
Tipo: Text Palavras-chave: PaHs; Contamination; Crassostrea gigas; Oyster.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3428.pdf
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Environment and immunomodulation : effects of the soluble fraction of Norwegian heavy fuel on the immune system of Sea bass, Dicentrarchus labrax ArchiMer
Bado-nilles, Anne; Quentin, Claire; Le Floch, Stéphane; Arzel, Jacqueline; Auffret, Michel; Gagnaire, Beatrice; Renault, Tristan; Thomas-guyon, Hélène.
Estuaries and coastal areas are often subjected to much pollution, including pesticides and polycyclic aromatic hydrocarbons (PAHs) produced by anthropic activities. Bay of Marennes-Oléron is an estuarine coastal zone in which there is a high level of aquacultural production and this has adverse effects in aquatic organisms. In fact, PAHs, which are widely spread throughout aquatic ecosystems, have a low polarity and so there is a clear tendency to bioaccumulate in marine organisms (1). The harmful effects of such contaminants on the organism's immune system, leading to a decreased resistance to pathogens, is a relatively new area of research and it still poorly understood. The aim of the present work was to detect the impact in vivo of PAHs on the immune...
Tipo: Text Palavras-chave: PaHs; Fish; Contamination; Dicentrarchus labrax.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3427.pdf
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Occurrence of plastic particles in procellariiforms, south of São Paulo state (Brazil) BABT
Barbieri,Edison.
Seabirds ingest plastic particles floating on the surface of the world's oceans. The birds can ingest plastic particles that they mistakenly identify as prey items. Alternatively, plastics can be taken up in the stomach contents of prey species. Plastic is often passed from parents to chicks in regurgitated food. In this study, individual petrels and albatrosses brought in by the tide onto Ilha Comprida beach between January 2000 and December 2002 were collected. Ilha Comprida, a barrier island in southern São Paulo, Brazil, was traversed by car along a transect of 70 kilometers. A total of 110 birds of 10 species were collected and 64.54% had plastic particles in their stomach. Frequencies of occurrence were Macronectes giganteus (64.28%), Thalassarche...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazil; Contamination; Ilha Comprida; Plastic; Seabirds; Procellariiformes.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200011
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Pesticide contamination of the coastline of Martinique ArchiMer
Bocquene, Gilles; Franco, Alain.
In January and February 2002, the presence of certain agricultural pesticides throughout the coastline of the Caribbean island of Martinique was investigated. The tropical climate of the French West Indies is suitable for banana production, which requires intensive use of pesticides. An inventory of all pesticides used on the island (compounds and tonnage) was compiled. Surveys and analyses revealed the presence of pesticides in the plumes of seven rivers. The organochlorine chlordecone and metabolites of aldicarb were detected at nearly all of the monitored sites, even though the use of chlordecone has been prohibited since 1993. Two triazines (ametryn and simazine) were also identified. The concentrations of carbamates and triazines detected in the water...
Tipo: Text Palavras-chave: Aldicarb; Chlordecone; Triazines; Pesticides; Contamination; Martinique Island.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-879.pdf
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High performance liquid chromatographic screening and gas chromatography-nass spectrometry confirmation of tebuthiuron residues in drinking water. Infoteca-e
BONATO, P. S.; LANCHOTE, V. L.; DREOSSI, A. de C..
Tebuthiuron (N-[5-(1,1-dimethylethyl)-1,3,4-thiadiazol-2-yl]-N,N-dimethylurea) is a phenylurea herbicide used in sugar cane culture for the pre- and post-emergence control of weeds [1,2]. Analysis of tebuthiuron (Figure 1) and of other phenylurea herbicides in environmental samples can be performed by HPLC [3-10], or by gas chromatography using selective detectors such as nitrogen-prosphorus detector (NPD), eletron-capture detector (ECD), or a mass spectrometer (MS) [11-16]. Although Loh et al [17] reported that the use of temperatures above 280oC permits quantitative tebuthiuron breakdown, thus permitting the analysis of the products of decomposition formed, methods based on gas chromatography usually involve a derivatization step to permit analysis of...
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E) Palavras-chave: Analysis; Contamination; Residues; Calibration; Environmental impact; Groundwater; Herbicides; Quality control.
Ano: 1999 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/13136
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Growth, condition and metal concentration in juveniles of two Diplodus species in ports ArchiMer
Bouchoucha, Marc; Brach-papa, Christophe; Gonzalez, Jean-louis; Lenfant, P.; Darnaude, A. M..
High abundances of juvenile fish in certain ports suggest they might provide alternative nursery habitats for several species. To further investigate this possibility, post-settlement growth, metal uptake and body condition were estimated in 127 juveniles of two seabream species, collected in 2014–15, inside and outside the highly polluted ports of the Bay of Toulon. This showed that differences in local pollution levels (here in Hg, Cu, Pb and Zn) are not consistently mirrored within fish flesh. Muscle metal concentrations, below sanitary thresholds for both species, were higher in ports for Cu, Pb and V only. Otherwise, fish muscle composition principally differed by species or by year. Juvenile growth and condition were equivalent at all sites. Higher...
Tipo: Text Palavras-chave: Coastal areas; Nursery habitats; Fish; Contamination; Otoliths.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00408/51932/52609.pdf
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Otolith fingerprints as natural tags to identify juvenile fish life in ports ArchiMer
Bouchoucha, Marc; Pecheyran, C.; Gonzalez, Jean-louis; Lenfant, P.; Darnaude, A. M..
The construction of ports has caused substantial habitat destruction in coastal areas previously used as nursery grounds by many fish species, with consequences to fish stocks. These artificial coastal areas might provide alternative nursery habitats for several species for juvenile fish abundances and growth in ports, although their contribution to adult stocks had never been estimated. The variability of otolith composition in the juveniles of two Diplodus species was investigated in three contrasting port areas and two adjacent coastal juvenile habitats of the Bay of Toulon (northwestern Mediterranean) in order to determine the possible use of otolith fingerprints as natural tags for the identification of juvenile fishes in ports. The global accuracy of...
Tipo: Text Palavras-chave: Coastal areas; Nursery habitats; Fish; LA-ICPMS; Contamination.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00449/56051/57571.pdf
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Toxicity and motor changes in Africanized honey bees (Apis mellifera L.) exposed to fipronil and imidacloprid Anais da ABC (AABC)
BOVI,THAÍS S.; ZALUSKI,RODRIGO; ORSI,RICARDO O..
ABSTRACT This study evaluated the in vitro toxicity and motor activity changes in African-derived adult honey bees (Apis mellifera L.) exposed to lethal or sublethal doses of the insecticides fipronil and imidacloprid. Mortality of bees was assessed to determine the ingestion and contact lethal dose for 24 h using probit analysis. Motor activities in bees exposed to lethal (LD50) and sublethal doses (1/500th of the lethal dose) of both insecticides were evaluated in a behavioral observation box at 1 and 4 h. Ingestion and contact lethal doses of fipronil were 0.2316 ? 0.0626 and 0.0080 ? 0.0021 μg/bee, respectively. Ingestion and contact lethal doses of imidacloprid were 0.1079 ? 0.0375 and 0.0308 ? 0.0218 μg/bee, respectively. Motor function of bees...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Environment; Pesticides; Pollinators; Contamination; Phenylpyrazoles; Neonicotinoids.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100239
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Bacterial contamination in milk kitchens in pediatric hospitals in Salvador, Brazil BJID
Cairo,Romilda Castro; Silva,Luciana Rodrigues; Andrade,Carol Ferreira de; Barberino,Maria Goreth de Andrade; Bandeira,Antônio Carlos; Santos,Kleber Pimentel; Diniz-Santos,Daniel R..
Milk may represent an important source of infectious agents to hospitalized pediatric patients. To describe the bacterial microflora isolated from the hands, stools, pharynx of all workers at milk kitchens in pediatric hospitals in the city of Salvador, Brazil, as well as in the formulas prepared by them, we carried out this cross-sectional study with all 91 workers from the 20 milk kitchens of all the public and private hospitals in Salvador, Brazil. Hand and pharynx swabs and stool samples were collected from all workers, as well as samples of the milk and formulas delivered by the kitchens. All samples were cultured for the detection of pathogenic and non-pathogenic bacteria. Pathogenic bacteria were isolated from 20 (22.0%) and 8 (8.8%) cultures of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteria; Microflora; Hands; Faeces; Pharynx; Milk; Contamination; Hospital; Children.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000300011
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Detection of transgenic events in maize using immunochromatographic strip test and conventional PCR Ciência e Agrotecnologia
Cantelmo,Narjara Fonseca; Von Pinho,Édila Vilela de Resende; Von Pinho,Renzo Garcia; Von Pinho,Iolanda Vilela; Nascimento,Micaela Sandim.
With the growth in the transgenic market, fast and economically viable methodologies are necessary for undertaking transgene detection tests, both for identification of contamination in seeds and in grain. Seeds from commercial conventional GNZ 2004, and transgenic VT-Pro (MON89034), Roundup Ready (NK603) and Herculex (TC1507) maize cultivars were used. In order to simulate different levels of contamination, the transgenic seeds were mixed with conventional seeds at levels of 0.2%, 0.4%, 1.0% and 1.6% for VT-Pro, and 0.2%, 0.5%, 0.8% and 1.2% for Roundup Ready and Herculex. The lateral flow membrane strip test was performed in the whole seed, endosperm and embryo. For evaluation of the specificity of the technique in detection of the TC1507 event by means...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fast testing; Contamination; Specificity; Transgenesis.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542013000500003
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